| RQP74014 | |
| I. Background | |
| The particles contain a firefly luciferase gene driven by the human IL-2 promoter (Figure 1). After transduction, activation of the IL-2 signaling pathway in the target cells can be monitored by measuring the luciferase activity. | |
| II. Description | |
| The IL-2 Promoter Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. | |
| III. Introduction | |
| Host Cell: | Jurkat |
| Expressed gene: | IL-2-Luciferase |
| Stability: | 32 passages |
| Synonym(s): | IL-2 Promoter reporter, lentivirus reporter, HIV reporter, VSV-G reporter |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | • Screen for activators or inhibitors of IL-2 signaling pathway in transduced target cells • Generation of IL-2 promoter luciferase reporter stable cell line |
| IV. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Peripheral blood |
| Disease: | Acute T cell leukemia |
| Morphology: | Lymphoblast |
| Growth Properties: | Suspension |
| Ⅴ. Representative Data | |
Figure 1. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. | |
| |
Figure 2. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. Jurkat-IL-2 Luciferase Reporter cells were stimulated by Ionomycin, the EC50 was 8.31ng/ml. | |
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