RQP74062 | |
I. Background | |
Human CD137 (4-1BB; TNFRS9) is an inducible costimulatory molecule that activates T cells. CD137-CD137L mediated signaling has been shown to be important for proliferation, effector functions and survival of T cells. CD137 is also expressed in NK and NKT cells. Antibodies targeting CD137 activation in immune cells have demonstrated potent anti-tumor effects in cancer patients. | |
II. Introduction | |
Host Cell: | NFkB reporter Jurkat cells |
Expressed gene: | 41BB |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Synonym(s): | 4-1BB, CD137 |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | RPMI-1640+10%FBS+800ug/ml hygromycin+1ug/ml puromycin |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
Application(s): | Functional(Report Gene) Assay |
III. Description of Host Cell Line | |
Organism: | Homo sapiens, human |
Tissue: | Peripheral blood |
Disease: | Acute T cell leukemia |
Morphology: | Lymphoblast |
Growth Properties: | Suspension |
IV. Representative Data | |
Figure 1. Recombinant 41BB/NFkB-Luc/Jurkat constitutively expressing 41BB. | |
Figure 2. Detect Luciferase assay by Ultra Luciferase Detection Kit RQPH0001(we strongly suggest to purchase from Reqbio). 4-1BB/Jurkat (C43) NFkB-Luc report assay stimulated by 4-1BBL. | |