| RQP74014 | |
| I. Background | |
| The particles contain a firefly luciferase gene driven by the human IL-2 promoter (Figure 1). After transduction, activation of the IL-2 signaling pathway in the target cells can be monitored by measuring the luciferase activity. | |
| II. Description | |
| The IL-2 Promoter Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. | |
| III. Introduction | |
| Host Cell: | Jurkat |
| Expressed gene: | IL-2-Luciferase |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | IL-2 Promoter reporter, lentivirus reporter, HIV reporter, VSV-G reporter |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+1ug/ml puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Peripheral blood |
| Disease: | Acute T cell leukemia |
| Morphology: | Lymphoblast |
| Growth Properties: | Suspension |
| Ⅴ. Representative Data | |
Figure 1. Response of IL -2-Luciferase Reporter Jurkat Cell Line (C29) to Raji TCR Activator Cell Line. | |
Figure 2. Dose Response of Recombinant hCTLA4-Fc in IL2 Promoter Reporter-Jurkat Cells with TCR Activitor Raji Cells.
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Figure 3. Detect Luciferase assay by Ultra Luciferase Detection Kit RQPH0001（we strongly suggest to purchase from Reqbio). Jurkat-IL-2 Luciferase Reporter cells were stimulated by anti-CD3, the EC50 was 8.31ng/ml. | |
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