RQP74031 | |
I. Description | |
NF-kB luciferase reporter construct is stably integrated into the genome of Jurkat T- cells. The firefly luciferase gene is controlled by 4 copies of NF-kB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-kB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene. | |
II. Introduction | |
Host Cell: | Jurkat |
Expressed gene: | NF-kB Luciferase |
Stability: | 32 passages |
Synonym(s): | Nfkb, nf-kb reporter, nfkb report, nf-kb jurkat |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | 1640+10%FBS+200ug/ml hygromycin |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
Application(s): | The NF-kB-luciferase/Jurkat cell line is suitable for monitoring the activity of NF-?B signaling in response to stimulants, and establishing cell-based screens for inhibitors that target specific NF- ?B-stimulating molecules. |
III. Description of Host Cell Line | |
Organism: | Homo sapiens, human |
Tissue: | Peripheral blood |
Disease: | Acute T cell leukemia |
Morphology: | Lymphoblast |
Growth Properties: | Suspension |
IV. Representative Data | |
Figure 1. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. Jurkat-NF-kB Luciferase Reporter cells were stimulated by Forsklin, the S/B was 12.43-fold. |