RQP74030 | |
I. Background | |
The MAPK/ERK signaling pathway is a major participant in the regulation of cell growth and differentiation. It can be activated by various extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, MEK1/2 phosphorylate and activate ERK1/2. The activated ERK translocates to the nucleus where it phosphorylates and activates transcription factors. The TCFs (Ternary Complex Factors), including Elk1, are among the best-characterized transcription factor substrates of ERK. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes. | |
II. Description | |
The SRE Reporter- HEK293 cell line is designed for monitoring the activity of the MAPK/ERK signaling pathway. The SRE Reporter-HEK293 cell line contains a firefly luciferase gene under the control of SRE responsive elements stably integrated into HEK293 cells, resulting in an ERK pathway-responsive reporter cell line. | |
III. Introduction | |
Host Cell: | HEK293 |
Expressed gene: | SRE-Luciferase |
Stability: | 32 passages |
Synonym(s): | SRE cell line, MAPK reporter, ERK reporter |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | DMEM+10%FBS+200ug/ml hygromycin |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
Application(s): | · Monitor MAPK/ERK signaling pathway activity and SRF-mediated activity. · Screen for activators or inhibitors of the MAPK/ERK signaling pathway. |
IV. Description of Host Cell Line | |
Organism: | Homo sapiens, human |
Tissue: | Embryonic kidney |
Disease: | Normal |
Morphology: | Epitheloid cell |
Growth Properties: | Adherent |
Ⅴ. Representative Data | |
Figure 1. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. HEK293/SRE Luciferase Reporter cells were stimulated by EGF, the S/B was 10-fold. |