Detailed introduction

The pan-tumor large panel standard "Panel-Ref ™ gDNA All-in-One Reference Standard" is a reference, quality control, and standard set for large panel genes, which can be used for QC verification of the detection process. Including accuracy, repeatability, detection limit, etc., the basic information is as follows:

name:	Panel-Ref^® gDNA All-in-One Reference Standard
Article number:	RQP90012
Product form:	gDNA
Verify the gene:	>400个
Verification site:	>700个
Corresponding dbSNP ID:	>400个
Corresponding Cosmic ID:	>570个
Verification platform:	NGS-Panel、DdPCR and WES 500X
% AF range:	1-100%
specification:	1ug/vial*1vial
Storage temperature:	4°C
Shelf life:	36 months
Data download:	Please download it in the "Product Database"
MSI Inspection Data：
Product Features:
1. The sample comes from a clinically established cell line, which is very close to the characteristics of clinical patients;

2. Pan-cancer species, involving a variety of tumor-related genes: lung cancer, breast cancer, colorectal cancer, pancreatic cancer, hematological tumors, gastric cancer, etc. Some genes are listed as follows:

Table 1. Partial display of pan-tumor-related targeting genes

3. Full chromosome coverage, except for the Y chromosome, all other chromosomes are involved;

Figure 1. Number of sites on different chromosome numbers (validated sites)

4. There are many types of mutation: involving SNP, deletion, insertion, CNV, etc .;

Figure 2. Number of sites with different types of mutations (verified sites)

5. Full coverage of mutation frequency, from 1% to 100%, all sections are involved;

Figure 3. Number of sites with different mutation frequencies (verified sites)

6. Mutation type full coverage, UTR region, intron, exon, missense mutation, synonymous mutation, donor mutation, etc .;

Figure 4. Number of loci for mutation subdivisions (validated loci)

7. Many sites, more than 680 sites, all with corresponding Cosmic ID or / and dbSNP ID annotations;

Figure 5. Number of sites annotated with dbSNP ID and Cosmic ID (verified sites)

8. Multi-platform verification, accurate and reliable data;
8.1 Among them, 1000x panel verified 16 sites, and panel is a product of a well-known commercial company;

Table 2. 16 sites verified by 1000x NGS-Panel kit

8.2 DdPCR verification of 8 sites, Bio-Rad QX200 and Bio-Rad original reagents

Table 3. Information of 8 sites verified by DdPCR (bio-rad) kit

8.3 500X WES verification of all sites, IDT xGenExome Research Panel v1.0 capture, HiSeq X-TEN sequencing

Table 4. Information of 400 genes verified by 500X WES

9. Gene coverage science, more than 400 genes, covering many well-known panel designs at home and abroad;

Table 5. Comparison of gene coverage

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