RQP30001L | |
I. Introduction | |
Host Cell: | HT-29 (TP53, p.R273H) |
Expressed gene: | Luciferase |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | McCoy's 5a+10%FBS+1ug/ml puromycin |
Mycoplasma Status: | Negative |
Storage: | Liquid nitrogen |
II. Description of Host Cell Line | |
Organism: | Homo sapiens, human |
Tissue: | Colo |
Disease: | Colorectal adenocarcinoma |
Morphology: | Epithelial |
Growth Properties: | Adherent |
III. Representative Data | |
Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System.. | |
IV. Stablity Test | |
Figure. Stablity test results. HT-29-Luc cells showed at least 32 passage stability with similar luminescence unit. |