RQP73236 | |
| I. Introduction | |
Cell Line Name： | c-Kit D820Y/BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Application: | Anti-proliferation assay and PD assay |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS |
Mycoplasma Status: | Negative |
| II.Background | |
KIT D820Y lies within the protein kinase domain of the Kit protein (UniProt.org). D820Yresuts in constitutive phosphorylation of Kit and is transforming in cell culture (PMID: 19035443, PMID: 11984533), and has also been identified as a secondary mutation associated with imatinib-resistance (PMID: 18488160). | |
| III. Representative Data | |
1. WB of c-Kit D820Y/BaF3
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2.Sanger of c-Kit D820Y/BaF3
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3. Anti-proliferation assay
Figure 3. CTG Proliferation Assay of BaF3 c-KIT D820Y (C1). | |
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