RQP73314 | |
| I. Introduction | |
Cell Line Name： | c-Kit V560Del/BaF3 |
Host Cell: | BA/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Application: | Anti-proliferation assay and PD assay |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS |
Mycoplasma Status: | Negative |
| II.Background | |
| KIT V560del (also reported as V559del) results in the deletion of an amino acid in the juxtamembrane domain (exon 11) of the Kit protein at amino acid 560 (PMID: 12879016). V560del confers a gain of function to Kit, resulting in constitutive, ligand independent phosphorylation of Kit and activation of Akt in cell culture (PMID: 15236194, PMID: 22282465), and transformation of cultured cells (PMID: 19861435). | |
| III. Representative Data | |
1. WB of c-Kit V560Del/BaF3
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2. Sanger of c-Kit V560Del/BaF3
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3. Anti-proliferation assay
Figure 3. CTG Proliferation Assay of BaF3 c-KIT V560del (C7). | |
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