Detailed introduction

RQP73301
I. Introduction
Cell Line Name：	EML4-ALK V1 [I1171N/L1198H]]/BaF3
Host Cell:	Ba/F3
Stability:	16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Application:	Anti-proliferation assay and PD assay
Freeze Medium:	90% FBS+10% DMSO
Complete Culture Medium:	RPMI-1640+10%FBS
Mycoplasma Status:	Negative

II.Background
EML4-ALK results from the fusion of EML4 and ALK, resulting in constitutive kinase activity, transformation in cultured cells, and tumor activity in mouse models (PMID: 19064915, PMID: 17625570). EML4-ALK fusions have been associated with non-small cell lung cancer (PMID: 26755435).

III. Representative Data
1.WB of EML4-ALK V1 [I1171N/L1198H]]/BaF3
2. Sanger of EML4-ALK V1 [I1171N/L1198H]]/BaF3 Figure 2. EML4-ALK V1 [I1171N/L1198H]]/BaF3 Fusion Figure 3. EML4-ALK V1 [I1171N/L1198H]]/BaF3 L1198H Figure 4. EML4-ALK V1 [I1171N/L1198H]]/BaF3 I1171N
2. Anti-proliferation assay Figure 5. CTG Proliferation Assay of BaF3 EML4-ALK V1 I1171N/L1198H (C1).