| RQP73296 | |
| I. Introduction | |
Cell Line Name： | c-Kit A502_Y503dup/BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS |
Mycoplasma Status: | Negative |
II. Background | |
| KIT A502_Y503dup (also referred to as Y503_F504insAY) indicates the insertion of 2 duplicate amino acids, alanine (A)-502 through tyrosine (Y)-503, in the Ig-like C2-type domain 5 (exon 9) of the Kit protein . A502_Y503dup results in constitutive phosphorylation of Kit and is transforming in cell culture. | |
III. Representative Data | |
1. WB of c-Kit A502_Y503dup/BaF3
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2. Sanger of c-Kit A502_Y503dup/BaF3
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3. Anti-proliferation assay
Figure 4. CTG Proliferation Assay of BaF3 C-KIT A502_Y503dup Cells (C5). | |
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