RQP73295 | |
| I. Introduction | |
Cell Line Name： | AKT1 WT/BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Application: | Anti-proliferation assay and PD assay |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS |
Mycoplasma Status: | Negative |
| II.Background | |
AKT1, RAC-alpha serine/threonine-protein kinase, is a ubquitously expressed serine-threonine protein kinase, which regulates a variety of cell functions including, metabolism, growth, angiogenesis, cell proliferation and survival through PI3K signaling in response to extracellular signals (PMID: 23297823, PMID: 26698230). Akt1 mutations are observed in a variety of tumor types (PMID: 23134728), including breast cancer (PMID: 30212483), gastric cancer (PMID: 29714127), salivary carcinoma (PMID: 29682203), and overexpression has been observed in osteosarcoma (PMID: 29762834) and gastric cancer (PMID: 29278885). | |
| III. Representative Data | |
1.WB of AKT1 WT/BaF3
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2.Sanger of AKT1 WT/BaF3
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3. Anti-proliferation assay | |
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Figure 3. CTG Proliferation Assay of BaF3 AKT1 WT Cells (C2). | |
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