| RQP73271 | |
| I. Introduction | |
Cell Line Name： | EML4-ALK V1 [G1202R/L1196M] /BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Application: | Anti-proliferation assay and PD assay |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS+2ug/ml puromycin |
Mycoplasma Status: | Negative |
| II.Background | |
EML4-ALK results from the fusion of EML4 and ALK, resulting in constitutive kinase activity, transformation in cultured cells, and tumor activity in mouse models (PMID: 19064915, PMID: 17625570). EML4-ALK fusions have been associated with non-small cell lung cancer (PMID: 26755435). | |
| III. Representative Data | |
1. WB of EML4-ALK V1 [G1202R/L1196M] /BaF3 expression
Figure 1 . Protein Expression of ALK detected by antibody.
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2. Sanger of EML4-ALK V1 [G1202R/L1198F] /BaF3
Figure 2 .EML4-ALK V1 [G1202R/L1198F] /BaF3 Fusion
Figure 3 . EML4-ALK V1 [G1202R/L1198F] /BaF3 [G1202R/L1198F]
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3. Anti-proliferation assay
Figure 4 . CTG Proliferation Assay of BaF3 EML4-ALK V1 [G1202R/L1196M] (C1). | |
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