Cell Line Name：

Host Cell:

Ba/F3

Application:

Anti-proliferation assay and PD assay

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

RPMI-1640+10%FBS 

Mycoplasma Status:

Negative

KIT W557del results in the deletion of an amino acid in the juxtamembrane domain (exon 11) of the Kit protein at amino acids 557 (PMID: 12918066). W557del has not been characterized, however similar Kit exon 11 deletions are activating (PMID: 9438854, PMID: 9797363), and therefore, is predicted to lead to a gain of Kit protein function .

1. WB of c-Kit W557_K558del/BaF3

2.Sanger of c-Kit W557_K558del/BaF3

3. Anti-proliferation assay

Figure 3. CTG Proliferation Assay of BaF3 c-KIT W557_ K558del (C2).