Cell Line Name：

Host Cell:

Ba/F3

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

RPMI-1640+10%FBS+2ug/ml puromycin

Mycoplasma Status:

Negative

KIT N822K lies within the activation loop in the protein kinase domain of the Kit protein (PMID: 24205792). N822K results in constitutive activation of Kit, increased Erk1/2 and Stat3 phosphorylation, is transforming in culture (PMID: 24205792, PMID: 31484543), leads to mislocalization of Kit to endolysosomes (PMID: 31484543), and has been identified as a secondary mutation associated with imatinib-resistance (PMID: 18488160).

1.WB of   c-Kit N822K/BaF3

2. Sanger of c-Kit N822K/BaF3

3. Anti-proliferation assay

Figure 3. CTG Proliferation Assay of BaF3 c-KIT N822K(C4).