Cell Line Name：

Host Cell:

Ba/F3

Application:

Anti-proliferation assay and PD assay

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

RPMI-1640+10%FBS 

Mycoplasma Status:

Negative

KIT D816Y lies within the tyrosine kinase domain 2 of the Kit protein (PMID: 21640708). D816Y results in constitutive phosphorylation of Kit, activation of Akt and Mapk pathways in cultured cells (PMID: 21640708, PMID: 16397263), and confers resistance to imatinib in culture (PMID: 16397263).

1. WB of c-Kit D816Y/BaF3

2.Sanger of c-Kit D816Y/BaF3

3. Anti-proliferation assay

Figure 3. CTG Proliferation Assay of BaF3 c-KIT D816Y (C1).