| RQP73275 | |
| I. Introduction | |
Cell Line Name： | AKT1 E17K/BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS |
Mycoplasma Status: | Negative |
| II.Background | |
AKT1 E17K lies within the PH domain of the Akt1 protein (UniProt.org). E17K leads to constitutive activation of Akt1, activates downstream signaling, and is transforming in culture (PMID: 17611497, PMID: 29533785, PMID: 27147599, PMID: 26701849). | |
| III. Representative Data | |
1.WB of AKT1 E17K/BaF3
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2. Sanger of AKT1 E17K/BaF3
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3. Anti-proliferation assay | |
Figure 3. CTG Proliferation Assay of BaF3 AKT1 E17K Cells (C3) | |
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