c-Kit WT (SCF1 Dependent)/BaF3

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RQP73269
I. Introduction

Cell Line Name:

c-Kit  WT (SCF1 Dependent)/BaF3

Host Cell:

BA/F3

Stability:16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)

Application:

Anti-proliferation assay and PD assay

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

RPMI-1640+10%FBS+0.25ug/ml Recombinant Human SCF Protein (R&D)

Mycoplasma Status:

Negative

 
II.Background

Human c-Kit also known as KIT, PBT, CD117, Piebald Trait, SCFR, and Mast/Stem Cell Growth Factor Receptor, GenBank Accession No. NM_000222, a.a. 544-976 with N-terminal His-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW=53 kDa.

 
III. Representative Data

1. WB of c-Kit WT (SCF1 Dependent)/BaF3

CBP73269-1.jpg

2. Anti-proliferation assay

Figure 2. CTG Proliferation Assay of BaF3 c-KIT BaF3 Cells(C1)..