Cell Line Name：

Host Cell:

Ba/F3

Application:

Anti-proliferation assay and PD assay

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

RPMI-1640+10%FBS 

Mycoplasma Status:

Negative

CSF3R T615A lies within the extracellular domain proximal to the transmembrane domain of the Csf3r protein (PMID: 23656643). T615A is predicted to confer a gain of function to the Csf3r protein, as demonstrated by increased proliferation of transformed cells in culture (PMID: 23656643).

1.WB of  CSF3R T615A/BaF3

2. Anti-proliferation assay

Figure 2. Anti-proliferation assay of three reference compounds on the CSF3R T615A/BaF3.