c-Kit D816V/BaF3

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Detailed introduction

RQP73206
I. Introduction
Cell Line Name：	c-Kit D816V/BaF3
Host Cell:	Ba/F3
Stability:	16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Application:	Anti-proliferation assay and PD assay
Freeze Medium:	90% FBS+10% DMSO
Complete Culture Medium:	RPMI-1640+10%FBS+2ug/ml puromycin
Mycoplasma Status:	Negative

II.Background
Human cKit with D816V substitution, GenBank Accession No. NM_000222, a.a. 544-end with N-terminal His- tag, expressed in a Baculovirus infected Sf9 cell expression system. MW=53 kDa.

III. Representative Data
1.WB of c-Kit D816V/BaF3
2. Anti-proliferation assay

Figure 3. Anti-proliferation assay of three reference compounds on the c-Kit D816V/BaF3.