| RQP73220 | |
| I. Introduction | |
Cell Line Name： | EGFR H773_V774insAH/BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS |
Mycoplasma Status: | Negative |
| II.Background | |
EGFR H773_V774insAH results in the insertion of two amino acids within the drug-binding pocket in the protein kinase domain of the Egfr protein between amino acids 773 and 774 (UniProt.org, PMID: 23371856). H773_V774insAH has not been biochemically characterized, but is predicted to result in a gain of function based on the effect of other Egfr exon 20 insertion mutations (PMID: 24353160). | |
| III. Representative Data | |
1. WB of EGFR H773_V774insAH/BaF3
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