| RQP71380 | |
| I. Background | |
| CRTH-2 is a 7-transmembrane G-protein coupled receptor that is expressed by activated Th2 cells and eosinophils. | |
| II. Introduction | |
| Host Cell: | CHO |
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+5ug/ml puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): | Functional assays for CRTH2 receptor |
| Transducer: | Gi |
| Ⅲ. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| Ⅳ. Representative Data | |
Figure 1. Recombinant CRTH2(DP2)/CHO constitutively expressing CRTH2(DP2). | |
Figure 2. HTRF CAMP Assay with CRTH2(DP2) CHO Cells (C1). | |