C-C chemokine receptor type 5 (CCR5) is a G protein-coupled receptor and a co-receptor for the entry of human
immunodeficiency virus-1 (HIV-1) into cells. CCR5 chemokine receptor is involved in leucocytes chemotaxis to sites of
inflammation and plays an important role in the macrophages, T cells, and monocytes recruitment. The chemokine
ligands that bind to CCR5 are regulated on activation, normal T Cell expressed and secreted (RANTES) and macrophage inflammatory protein 1 alpha (MIP1α). GenScript’s human CCR5-expressing stable subline is guaranteed to function properly in the calcium flux assay.

CHO-K1

C-C chemokine receptor type 5, CCR5

Functional assay for CCR5 receptor

Concentration-dependent stimulation of intracellular calcium mobilization by Rantes in CCR5/Ga16/CHO and CHO cells.

Figure 1. Rantes-induced concentration-dependent stimulation of intracellular calcium mobilization in CCR5/Ga16/CHO and CHO cells. The cells were loaded with Calcium-4prior to stimulation with an CCR5 receptor agonist, Rantes. The intracellular calcium change was measured by FlexStation.